An alternative method for the decontamination of rats carrying Mycoplasma pulmonis without the use of germfree isolators |
| |
Authors: | A K Banerjee A F Angulo A A Polak-Vogelzang A M Kershof |
| |
Abstract: | Two strains of Lewis rat were successfully freed from Mycoplasma pulmonis infection by using a combination of oral treatment with oxytetracycline hydrochloride and obtaining young by hysterectomy. Laminar flow cabinets were used to perform hysterectomies on donor animals and for rearing hysterectomy-derived animals. After thorough microbiological examination the rats were brought to the breeding colony of the Laboratory Animal Centre. Periodic laboratory tests using both cultural and enzyme-linked immunosorbent assay methods showed that the animals have remained free from M. pulmonis for the last 3 years. |
| |
Keywords: | |
|