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烟草表皮细胞薄层培养系统中多种组织器官发生的研究
引用本文:许萍,王琦,陈小村,张丕方.烟草表皮细胞薄层培养系统中多种组织器官发生的研究[J].武汉植物学研究,1997,15(1):1-4.
作者姓名:许萍  王琦  陈小村  张丕方
作者单位:[1]复旦大学生物系 [2]美国伊阿华大学
基金项目:国家自然科学基金,国家教委博士点基金
摘    要:利用烟草表皮细胞薄层培养系统,研究了离体培养下表皮毛和气孔的发生,发现斜向分裂和不均等分裂是与脱分化细胞再分化相关的分裂方式,并观察了愈伤组织气孔的适应性分化现象,此外,在茎表皮细胞薄层上成功地发生了不定根,利用花茎表皮细胞薄层也在其愈伤组织上发生了花芽。

关 键 词:烟草  表皮细胞  薄层培养  器官发生
收稿时间:1995-9-24
修稿时间:1995-11-26

STUDY ON MORPHOGENESIS OF STUDY ON MORPHOGENESIS OF MULTI-TISSUE AND ORGANS IN THE TCL OF TOBACCO EPIDERMIS
Xu Ping,Wang Qi,Chen,Xiaocun,Zhang Pifang.STUDY ON MORPHOGENESIS OF STUDY ON MORPHOGENESIS OF MULTI-TISSUE AND ORGANS IN THE TCL OF TOBACCO EPIDERMIS[J].Journal of Wuhan Botanical Research,1997,15(1):1-4.
Authors:Xu Ping  Wang Qi  Chen  Xiaocun  Zhang Pifang
Institution:Department of Biology, Fudan University, Shanghai 200433
Abstract:Morphogenesis of epidermal hair and stoma was observed through establishing the Thin Cell Layer (TCL) culture system of tobacco epidermis. It was found that uneven and slant cell division was related to redifferentiation of dedifferentiated cells.And formation of stoma in calli from TCL showed the characteristics of adaption to enviroment while the cells redifferentiated. Besides,adventitious roots had been induced to regenerate successively. And floral bud differentiation was accomplished in TCL cultureof tobacco floral stems.
Keywords:Tobacco  Epidermal cells  TCL(thin cell layer)  Organgenesis
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