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中温螺旋藻新品系的选育研究
引用本文:殷春涛,胡鸿钧,李夜光,龚小敏,施定基.中温螺旋藻新品系的选育研究[J].武汉植物学研究,1997,15(3):250-254.
作者姓名:殷春涛  胡鸿钧  李夜光  龚小敏  施定基
作者单位:[1]中国科学院武汉植物研究所 [2]中国科学院植物研究所
摘    要:利用超声波处理钝顶螺旋藻出发品系NS-90020,获得单细胞螺旋藻;然后用NTG诱变筛选获得突变品系Sp-ALT1;比较出发品系和突变品系的一般形态和生理生化特征,出发品系和突变品系的一般形态有较大差异。与出发品系相比,突变品系藻丝体显著变短,螺旋数目大大减少。它们光合作用温度曲线的差异性表明,突变品系Sp-ALT1的最适放氧温度比出发品系低,用4℃低温处理这两个品系,突变品系比出发品系更能忍受低

关 键 词:钝顶螺旋藻  NTG  单细胞  突变品系
收稿时间:1997-2-3
修稿时间:1997-2-28

STUDIES ON MIDDLE TEMPERATURE STRAINS SELECTION OF SPIRULINA PLATENSIS
Yin Chuntaol Hu Hong inn'' Li Yeguang'' Gong xiaominl Shi Ding n''.STUDIES ON MIDDLE TEMPERATURE STRAINS SELECTION OF SPIRULINA PLATENSIS[J].Journal of Wuhan Botanical Research,1997,15(3):250-254.
Authors:Yin Chuntaol Hu Hong inn' Li Yeguang' Gong xiaominl Shi Ding n'
Institution:1. Wuhan Institute of Botany, The Chinese Academy of Sciences Wuhan 430074;2. Institute of Botany, The Chinese Academy of Sciences Beijing 100093
Abstract:Single cells of SPirulina platensis were made by treating the initial strain(Sp)NS-90020 with Ultrasonic Homogenizer, then the mutant, SP-ALT, were selected afterinducing these single cells with NTG. And their general morphology,physiological features and biochemical composition were studied comparatively. There are some morphological differences between the initiall strain and the mutant. The filaments of the mutant show much shorter lengh and fewer numbers of the helix than of the initial strain.The temperature curves of photosynthesis showed that the optimum temperature of oxygen-evolution of SP-ALT, was lower than that of the initial strain. When they weretreated at 4'C,the mutant could endure much lower temperature than the initial strain.These showed that SP-ALT, was a middle temperature strain. In addition,the protein inSP-ALT, increased by 9. 5% compared with the initial strain.
Keywords:Spirulina platensis  NTG  Single cell  Mutants
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