Nuclear DNA Content as an Index Character Discriminating Taxa in the Genus Petunia sensu Jussieu (Solanaceae) |
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| Authors: | Mishiba Kei-Ichiro; Ando Toshio; Mii Masahiro; Watanabe Hitoshi; Kokubun Hisashi; Hashimoto Goro; Marchesi Eduardo |
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| Institution: | Faculty of Horticulture, Chiba University, 648 Matsudo, Chiba Prefecture, 271-8510, Japan Centro de Pesquisas de História Natural, 618 Rua Jaime Ribeiro Wright, Itaquera, São Paulo, 08260-070, Brazil Facultad de Agronomia, Universidad de República, Garzón, Montevideo, 8780, Uruguay |
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| Abstract: | Nuclear DNA contents over the total range of the genus Petuniasensu Jussieu comprising 20 taxa of Petunia sensu Wijsman (2n = 2 x = 14) and 32 taxa of Calibrachoa(2 n = 2 x = 18) wereestimated by flow cytometry after staining the nuclei with propidiumiodide (PI) or 4',6-diamidino-2-phenylindole (DAPI). With respectto nuclear DNA content, taxa of Petunia sensu Wijsman seemedto be homogeneous (2C = 2.60 to 3.41 pg), but Calibrachoa taxawere clearly separated into two groups: (1)C. parviflora andC. pygmaea(1.56 to 1.91 pg); and (2) remaining members of Calibrachoa(2.84to 3.26 pg). Taxa of Petunia sensu Wijsman exhibited largerPI/DAPI ratios (relative fluorescence intensity with PI stainingto that with DAPI staining) than Calibrachoa species exceptC.parviflora and C. pygmaea. This suggests that Petunia sensuWijsman has nuclear DNA with more adenine-thymine rich regionsthan Calibrachoa. Copyright 2000 Annals of Botany Company Calibrachoa, 4',6-diamidino-2-phenylindole (DAPI), flow cytometry, nuclear DNA content,Petunia , PI/DAPI ratio, propidium iodide (PI), Solanaceae |
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