Multilocus enzyme electrophoretic analysis and DNA-DNA reassociation of strains designated Prevotella intermedia |
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| Authors: | HN Shah † SE Gharbia |
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| Institution: | *Department of Oral Biology, Faculty of Dentistry, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada B3H 3J5;†Department of Microbiology and Immunology, Faculty of Medicine, Dalhousie University, Halifax, Nova Scotia, Canada B3H 3J5 |
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| Abstract: | Strains of Prevotella intermedia which have been characterized previously by a variety of biochemical and chemical techniques were subjected to multilocus enzyme electrophoresis and DNA-DNA reassociation. Two separate groups of strains were discernible. One had high homology with the type strain ATCC 25611 DNA probe and electrophoretically fast migrating malate dehydrogenase (MDH) (3.8–4.0 cm) and glutamate dehydrogenase (GDH) (3.2–3.4 cm) bands. The other group of strains hybridized with the DNA probe of reference strain ATCC 33563 and possessed slower moving enzymes (MDH, 3.0 cm; GDH, 1.4–1.6 cm). These results indicate that strains currently identified as P. intermedia comprise at least two geno-species, and that the criteria used to define this species are inadequate. |
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