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Solid-state NMR triple-resonance backbone assignments in a protein |
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| Authors: | Wee Meng Tan Zhengtian Gu Ana Carolina Zeri Stanley J Opella |
| Institution: | (1) Department of Chemistry, University of Pennsylvania, Philadelphia, PA, 19104, U.S.A. |
| Abstract: | Triple-resonance solid-state NMR spectroscopy is demonstrated to sequentially assign the 13C and 15N amide backbone resonances of adjacent residues in an oriented protein sample. The observed 13C chemical shift frequency provides an orientational constraint complementary to those measured from the 1H and 15N amide resonances in double-resonance experiments. |
| Keywords: | coat protein filamentous bacteriophage PISEMA oriented samples resonance assignments solid-state NMR spectroscopy triple-resonance |
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