The effect of taurine on renal ischemia/reperfusion injury |
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Authors: | G. Guz E. Oz N. Lortlar N. N. Ulusu N. Nurlu B. Demirogullari S. Omeroglu S. Sert C. Karasu |
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Affiliation: | (1) Department of Nephrology, Gazi University Faculty of Medicine, Ankara, Turkey;(2) Department of Physiology, Gazi University Faculty of Medicine, Ankara, Turkey;(3) Department of Histology and Embryology, Gazi University Faculty of Medicine, Ankara, Turkey;(4) Department of Biochemistry, Hacettpe University Faculty of Medicine, Ankara, Turkey;(5) Department of Biochemistry, Gazi University Faculty of Medicine, Ankara, Turkey;(6) Department of Pediatric Surgery, Gazi University Faculty of Medicine, Ankara, Turkey;(7) Department of Transplantation Surgery, Gazi University Faculty of Medicine, Ankara, Turkey;(8) Department of Medical Pharmacology, Gazi University Faculty of Medicine, Ankara, Turkey |
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Abstract: | Summary. Ischemia-reperfusion (I/R) injury is one of the most common causes of renal dysfunction. Taurine is an endogenous antioxidant and a membrane-stabilizing, intracellular, free beta-amino acid. It has been demonstrated to have protective effects against I/R injuries to tissues other than kidney. The aim of this study was to determine whether taurine has a beneficial role in renal I/R injury. Forty Wistar-Albino rats were allocated into four groups as follows: sham, taurine, I/R, and I/R + taurine. Taurine 7.5 mg/kg was given intra-peritoneally to rats in the groups taurine and I/R + taurine. Renal I/R was achieved by occluding the renal arteries bilaterally for 40 min, followed by 6 h of reperfusion. Immediately thereafter, blood was drawn and tissue samples were harvested to measure 1) serum levels of BUN and creatinine; 2) serum and/or tissue levels of malondialdehyde (MDA), glutathione (GSH), glucose 6-phosphate dehydrogenase (G-6PD), 6-phosphogluconate dehydrogenase (6-PGD) and glutathione reductase (GSH-red); 3) renal morphology; and 4) immunohistochemical staining for P-selectin. Taurine administration reduced I/R-induced increases in serum BUN and creatinine, and serum and tissue MDA levels (p < 0.05). Additionally, taurine lessened the reductions in serum and tissue glutathione levels secondary to I/R (p < 0.05). Taurine also attenuated histopathologic evidence of renal injury, and reduced I/R-induced P-selectin immunoreactivity (p < 0.05). Overall, then, taurine administration appears to reduce the injurious effects of I/R on kidney. |
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Keywords: | : Taurine – Ischemia – Reperfusion – Kidney – Malondialdehyde and glutathione |
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