The development of a radioimmunoassay for formoterol |
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Authors: | K Yokoi K Murase Y Shiobara |
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Affiliation: | 1. Institute of Research and Development, Japan;1. Central Research Laboratories, Yamanouchi Pharmaceutical Co. Ltd., Azusawa 1-1-8, Itabashi-ku, Tokyo, Japan |
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Abstract: | The development of a radioimmunoassay (RIA) for the beta 2-stimulant formoterol is described. The sensitivity of the method is 0.1 ng/ml in plasma and urine, when a 1-ml sample is used. The cross-reactivity of the antiserum with formoterol glucuronide was 30%. Since formoterol is metabolized extensively to formoterol glucuronide in rats, dogs and humans, extraction with ethyl ether prior to the radioimmunoassay was carried out. Satisfactory agreement was obtained for levels of formoterol in plasma and urine when they were determined by RIA and gas chromatography-mass spectrometry. The concentration of formoterol was determined in dog plasma and human urine after oral administration of formoterol fumarate to dogs (61 mcg/kg) and humans (40 mcg). |
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