Styrene biosynthesis from glucose by engineered E. coli |
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Authors: | McKenna Rebekah Nielsen David R |
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Affiliation: | Chemical Engineering, Arizona State University, 501 E. Tyler Mall, ECG 301, Tempe, AZ 85287-6106, USA |
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Abstract: | Styrene is a large volume, commodity petrochemical with diverse commercial applications, including as a monomer building-block for the synthesis of many useful polymers. Here we demonstrate how, through the de novo design and development of a novel metabolic pathway, styrene can alternatively be synthesized from renewable substrates such as glucose. The conversion of endogenously synthesized l-phenylalanine to styrene was achieved by the co-expression of phenylalanine ammonia lyase and trans-cinnamate decarboxylase. Candidate isoenzymes for each step were screened from bacterial, yeast, and plant genetic sources. Finally, over-expression of PAL2 from Arabidopsis thaliana and FDC1 from Saccharomyces cerevisiae (originally classified as ferulate decarboxylase) in an l-phenylalanine over-producing Escherichia coli host led to the accumulation of up to 260 mg/L in shake flask cultures. Achievable titers already approach the styrene toxicity threshold (determined as ∼300 mg/L). To the best of our knowledge, this is the first report of microbial styrene production from sustainable feedstocks. |
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Keywords: | Styrene Phenylalanine ammonia lyase E. coli Cinnamic acid font-variant: small-caps" >l-Phenylalanine Aromatic |
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