A simple and rapid chromatographic method for the immobilization of acetylcholinesterase from electric eel. |
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| Authors: | R M Kothari M A Grafius D B Millar |
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| Affiliation: | Laboratory of Physical Biochemistry, Department of Environmental Biosciences, Naval Medical Research Institute, National Naval Medical Center, Bethesda, Maryland 20014 USA |
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| Abstract: | Acetylcholinesterase from electric eel is selectively immobilized on Amberlite IR-120 resin equilibrated with Al3+ ions. Immobilized acetylcholinesterase activity is stable at least for 85 days in the wet state at 10°C and for 180 days in the dry state at room temperature. Activity determinations in the presence of eserine sulfate, decamethonium bromide, quinidine sulfate and butyryl thiocholine iodide suggested that the immobilized enzyme exhibited essentially the same properties as did the free enzyme. |
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