Single-seed PCR of LMW glutenin genes to distinguish between durum wheat cultivars with good and poor technological properties |
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Authors: | Renato D'Ovidio |
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Affiliation: | (1) Dipartimento di Agrobiologia e Agrochimica, Università degli Studi della Tuscia, Via San Camillo de Lellis, 01100 Viterbo, Italy |
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Abstract: | Polymerase chain reaction (PCR) was used to amplify low-molecular-weight (LMW) glutenin sequences from genomic DNA extracted from a single germinating seed of several durum wheat genotypes. Electrophoretic analysis of PCR reactions showed the presence of amplified products characteristic of durum wheat cultivars with good and poor technological properties. This PCR-based approach is proposed as a very efficient and safe alternative to standard procedures for selecting durum wheat genotypes with good qualitative characteristics. |
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Keywords: | polymerase chain reaction LMW glutenin genes wheat quality |
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