| Vitex glabrata R.Br.悬浮培养细胞的生长条件优化及20-羟基蜕皮激素的生产 |
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| 引用本文： | Duangjai Sinlaparay,Preeyada Duanghaklang,Sanha Panichajakul. Vitex glabrata R.Br.悬浮培养细胞的生长条件优化及20-羟基蜕皮激素的生产[J]. 微生物学报, 2007, 23(6) |
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| 作者姓名： | Duangjai Sinlaparay Preeyada Duanghaklang Sanha Panichajakul |
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| 摘 要： | 研究了培养基、植物生长调节素以及接种量对Vitex glabrata R.Br.悬浮培养细胞的生长情况以及对20-羟基蜕皮激素形成的作用。当细胞在添加有2.0mg/L BAP (6-苯甲酸嘌呤)和1.0mg/L 2,4-D的Gamborg's B5培养基中培养时细胞生长和20-羟基蜕皮激素的形成达到了最高水平。当接种量为20％PCV（积压细胞体积）时观察到了20-羟基蜕皮激素的最高产量，大约是1.1mg/(L·d)。实验数据也表明当接种量增加到20％PCV时，产量提高了7倍。
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| 关 键 词： | 20-羟基蜕皮激素 悬浮培养 Vitex glabrata |
Optimization of Cell Growth and 20-Hydroxyecdysone Production in Cell Suspension Culture of Vitex glabrata R.Br. |
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| Duangjai Sinlaparay,Preeyada Duanghaklang and Sanha Panichajakul. Optimization of Cell Growth and 20-Hydroxyecdysone Production in Cell Suspension Culture of Vitex glabrata R.Br.[J]. Acta microbiologica Sinica, 2007, 23(6) |
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| Authors: | Duangjai Sinlaparay Preeyada Duanghaklang Sanha Panichajakul |
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| Affiliation: | Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002 Thailand;Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002 Thailand;Department of Biotechnology, Faculty of Technology, Khon Kaen University, Khon Kaen 40002 Thailand |
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| Abstract: | |
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| Keywords: | 20-hydroxyecdysone suspension cultures Vitex glabrata |
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