Quantification of single-stranded nucleic acid and oligonucleotide interactions with metal ions by affinity capillary electrophoresis: part I |
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Authors: | Alexandra R. Stettler Valérie Chaurin Edwin C. Constable Catherine E. Housecroft Maria A. Schwarz |
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Affiliation: | (1) Department of Chemistry, University of Basel, Spitalstrasse 51, 4056 Basel, Switzerland |
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Abstract: | The interactions between oligonucleotides and inorganic cations have been measured by capillary zone electrophoresis. With increasing concentrations of divalent cations (Ca2+, Mg2+, Mn2+ and Ni2+) in the running buffer, the migration behavior was evaluated by calculation of the binding constants. Besides these fundamental studies of binding equilibria, different buffer components, tris(hydroxymethyl)aminomethane and 3-(N-morpholino)propanesulfonic acid, have been investigated and their effects on metal ion binding quantified. |
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Keywords: | Affinity capillary electrophoresis Buffer Oligonucleotide Metal cation |
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