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| C3d-M28增强伪狂犬病毒gC基因体液免疫 | |
| 引用本文： | 樊惠英,刘中勇,佟铁铸,刘星,郭爱珍. C3d-M28增强伪狂犬病毒gC基因体液免疫[J]. 生物工程学报, 2009, 25(7): 987-992 |
| 作者姓名： | 樊惠英 刘中勇 佟铁铸 刘星 郭爱珍 |
| 作者单位： | 1. 华南农业大学兽医学院,广州,528427 2. 广东省出入境检验检疫局技术中心,广州,541004 3. 惠州市出入境检验检疫局技术中心,惠州,516006;华中农业大学畜牧兽医学院,武汉,430070 4. 惠州市出入境检验检疫局技术中心,惠州,516006 5. 华中农业大学畜牧兽医学院,武汉,430070 |
| 基金项目： | 国家自然科学基金项目(No.30800826)资助~~ |
| 摘 要： | 研究补体C3d的受体结合功能区(M28)对伪狂犬病毒gC基因DNA疫苗免疫增强作用。将4拷贝的M28基因与伪狂犬病毒gC基因串联后,克隆到载体pcDNA3.1中,构建融合表达的重组质粒(sgC-M284)。BALB/c小鼠免疫试验表明,sgC-M284免疫组比单独表达伪狂犬病毒gC蛋白的重组质粒(sgC)免疫组产生的ELISA抗体高17倍,对致死剂量(316LD50)伪狂犬病毒攻毒的保护率提高了63%。gC基因与M28基因融合表达诱导产生的IL-4水平接近了伪狂犬灭活疫苗免疫组的产生的IL-4水平,显著增强了基于Th2途径的体液免疫反应。 |
| 关 键 词： | 补体C3d gC糖蛋白 伪狂犬病毒 C3d受体结合功能区（M28） 分子免疫佐剂 |
| 收稿时间： | 2009-01-19 |
C3d-M28 enhanced DNA vaccination induced humoral immune response to glycoprotein C of pseudorabies virus |
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| Huiying Fan,Zhongyong Liu,Tiezhu Tong,Xing Liu and Aizhen Guo. C3d-M28 enhanced DNA vaccination induced humoral immune response to glycoprotein C of pseudorabies virus[J]. Chinese journal of biotechnology, 2009, 25(7): 987-992 | |
| Authors: | Huiying Fan Zhongyong Liu Tiezhu Tong Xing Liu Aizhen Guo |
| Affiliation: | College of Veterinary Medicine, South China Agricultural University, Guangzhou 528427, China;Guangdong Entre-exit Inspection and Quarantine Bureau, Guangzhou 541004, China;Huizhou Entre-exit Inspection and Quarantine Bureau, Huizhou 516006, China; College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China;Huizhou Entre-exit Inspection and Quarantine Bureau, Huizhou 516006, China;College of Veterinary Medicine, Huazhong Agricultural University, Wuhan 430070, China |
| Abstract: | We studied the immunogenicity of pseudorabies virus gC DNA vaccination by fusing the murine complement C3d receptor binding domain. First, pseudorabies virus gC gene was linked to four copies of C3d receptor binding domain (M284), and then cloned into the vector pcDNA3.1 to construct the recombinant plasmid sgC-M284. Through the experiment of immunized BALB/c mice, we found that the enzyme linked immunosorbent assay (ELISA) antibody titer for sgC-M284 was 17-fold higher than that for sgC alone, and protecti... |
| Keywords: | C3d gC protein pseudorabies virus complement C3d receptor binding domain M28 molecular adjuvants |
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