Erythrosin B as an effective inhibitor of electrogenic H+ extrusion |
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Authors: | M C COCUCCI E MARRÉ |
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Institution: | Centro di Studio del CNR per la Biologia Cellulare e Molecolare delle Piante, Laboratorio di Fisiologia Vegetale dell'Universita' di Milano. Dipartimento di Biologia, via Celoria 26, 20133 Milano |
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Abstract: | Abstract In 24-h-genninaled radish seedlings erythrosin B (EB), an effective inhibitor of microsomal as well as of partially purified vanadate-sensitive ATPase markedly inhibited the basal and the FC-stimulated proton extrusion, and induced a rapid depolarization of FC-hyperpolarized trans-membrane electric potential (PD) without causing any significant change of ATP level. The effects of EB on H+ extrusion were partially additive with those of vanadatc, another inhibitor of plasma membrane H+-ATPase. These effects are interpreted as due to a direct inhibition by EB on plasma membrane H+-ATPase involved in H+ electrogenic transport in the higher plants. |
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Keywords: | erythrosin B H+ extrusion transmembrane electric potential fusicoccin plasmalemma ATPase |
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