MZF6D, a novel KRAB zinc-finger gene expressed exclusively in meiotic male germ cells |
| |
Authors: | Looman Camilla Mark Charlotta Abrink Magnus Hellman Lars |
| |
Affiliation: | Department of Cell and Molecular Biology, Uppsala University, Uppsala, Sweden. |
| |
Abstract: | Spermatogenesis takes place in the seminiferous tubule in the testes and culminates in the production of spermatozoa (male gametes). Here we report the identification of a novel mouse zinc-finger gene, MZF6D, which is selectively expressed in meiotic spermatocytes. The MZF6D protein contains an N-terminally located repressor domain, a KRAB domain, followed by at least seven successive Krüppel zinc-finger motifs. The KRAB domain of MZF6D, which consists of a KRAB A box and the newly identified KRAB C box, has previously been shown to interact with TIF1beta, which is the common corepressor of all KRAB zinc-finger proteins. Northern blot analysis shows that the expression of MZF6D is restricted to testes. This was confirmed by RT-PCR analysis of a panel of mouse tissues. In situ hybridization of sections from adult mouse testes localizes the expression to meiotic spermatocytes, suggesting a specific role for MZF6D in the regulation of spermatogenesis. |
| |
Keywords: | |
本文献已被 PubMed 等数据库收录! |
|