Thidiazuron Induced Multiple Shoot Induction and Plant Regeneration from Cotyledonary Explants of Mulberry |
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Authors: | T Dennis Thomas |
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Institution: | (1) Post Graduate and Research Department of Botany, St. Thomas College, Pala, Arunapuram-, 686574 Kottayam (Dt), Kerala, India |
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Abstract: | A rapid micropropagation protocol through induced multiple shoots from the cotyledonary explant of mulberry (Morus alba L) is described. The highest number of shoots (20.3) was obtained when explants from 14-d-old embryos were cultured on Murashige
and Skoog (MS) medium supplemented with 7 μM thidiazuron for 45 d. Of the three cultivars used, cv. S-36 was the best followed
by cv. K-2 and S-1. The shoots were transferred to MS medium supplemented with 5 μM 6-benzylaminopurine for elongation. The
elongated shoots were rooted on half strength MS medium containing 1 – 7 μM indole 3-butyric acid or 1-naphthalene acetic
acid. The rooted plants were transplanted to soil with 90 % success. The emerged shoot primordia probably initiated from the
pre-existing meristems since the shoot bud show definite vascular connection to the major vascular tissue.
This revised version was published online in July 2006 with corrections to the Cover Date. |
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Keywords: | acclimatization growth regulators Morus alba |
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