A facile method to synthesize histones with posttranslational modification mimics |
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| Authors: | Wang Zhiyong U Wang Yane-Shih Pai Pei-Jing Russell William K Russell David H Liu Wenshe R |
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| Affiliation: | Department of Chemistry, Texas A&M University , College Station, Texas 77843, United States. |
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| Abstract: | Using an evolved pyrrolysyl-tRNA synthetase-tRNA(Pyl) pair, a Se-alkylselenocysteine was genetically incorporated into histone H3 with a high protein expression yield. Quantitative oxidative elimination of Se-alkylselenocysteine followed by Michael addition reactions with various thiol nucleophiles generated biologically active mimics of H3 with posttranslational modifications including lysine methylation, lysine acetylation, and serine phosphorylation. |
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