Unusual denaturation properties of vicilin from Cajanus cajan |
| |
| Authors: | Y R Mawal M R Mawal P K Ranjekar |
| |
| Affiliation: | Division of Biochemical Sciences, National Chemical Laboratory, (Maharashtra), India. |
| |
| Abstract: | Pigeonpea (Cajanus cajan) vicilin (Mr 190 kD) holoprotein contains 2 subunits and the N-terminal amino acid sequence is Gly-Ala-Arg-Val-Asp-Gln-Glu for purified vicilin subunit 1 (Mr 72 kD) and Thr-Thr-Cys-Met-Glu-Ser-Gly for purified vicilin subunit 2 (Mr 57 kD). Circular dichroism spectra of vicilin indicate the occurrence of a predominant beta- pleated sheet structure. The fluorescence studies of vicilin reveal its unusual stability to 8 M urea and 6 M guanidine HCl. |
| |
| Keywords: | |
|
|
