EBP, a program for protein identification using multiple tandem mass spectrometry datasets |
| |
Authors: | Price Thomas S Lucitt Margaret B Wu Weichen Austin David J Pizarro Angel Yocum Anastasia K Blair Ian A FitzGerald Garret A Grosser Tilo |
| |
Institution: | Institute for Translational Medicine and Therapeutics, University of Pennsylvania, Philadelphia, Pennsylvania 19104, USA. |
| |
Abstract: | MS/MS combined with database search methods can identify the proteins present in complex mixtures. High throughput methods that infer probable peptide sequences from enzymatically digested protein samples create a challenge in how best to aggregate the evidence for candidate proteins. Typically the results of multiple technical and/or biological replicate experiments must be combined to maximize sensitivity. We present a statistical method for estimating probabilities of protein expression that integrates peptide sequence identifications from multiple search algorithms and replicate experimental runs. The method was applied to create a repository of 797 non-homologous zebrafish (Danio rerio) proteins, at an empirically validated false identification rate under 1%, as a resource for the development of targeted quantitative proteomics assays. We have implemented this statistical method as an analytic module that can be integrated with an existing suite of open-source proteomics software. |
| |
Keywords: | |
本文献已被 ScienceDirect PubMed 等数据库收录! |
|