A Biosurfactant-Producing Pseudomonas aeruginosa Strain |
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Authors: | Turkovskaya O. V. Dmitrieva T. V. Muratova A. Yu. |
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Affiliation: | (1) Institute of Biochemistry and Physiology of Plants and Microorganisms, Russian Academy of Sciences, pr. Entuziastov 13, Saratov, 410015, Russia |
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Abstract: | A Pseudomonas aeruginosa strain producing an extracellular surfactant (biosurfactant) was isolated. The growth of this strain, referred to as 50.3, on a mineral glycerol-containing medium produces an emulsifying activity (60%) and decreases the surface tension of the culture liquid by a factor of 2.8 (to 25 mN/m). The optimum conditions for its growth and production of biosurfactants are intense aeration, pH 7.0–8.0, and the presence of Mg2+. The optimum biosurfactant properties were achieved when glucose was used as the only source of carbon and energy and NH4Cl was used as a source of nitrogen. The biosurfactant was isolated from the culture liquid by extraction and precipitation. |
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