Validation of reference genes in the feline endometrium |
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| Institution: | 1. Clinic of Small Animal Medicine, LMU University of Munich, Veterinaerstr. 13, 80539 Munich, Germany;2. Molecular Diagnostic Unit, Langford Veterinary Services, University of Bristol, Langford, Bristol BS40?5DU, UK;3. Clinic for Ruminants with Ambulatory Clinic and Herd Health Management, LMU University of Munich, Sonnenstr. 16, 85764 Oberschleissheim, Germany |
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| Abstract: | The aim of the study was to find the most stable reference genes from: ACTB, GAPDH, RPL30, CYC, RPL17, RPS7 and YWHAZ in the feline endometrium. Three free software packages, geNorm, NormFinder and BestKeeper were used. In geNorm analysis, the most stable gene was RPS7 (at a primer concentration 1000 nM) or YWHAZ (500 and 250 nM). According to NormFinder and BestKeeper, ACTB (at all examined primer concentrations) followed by RPS7 and CYC were the most stable genes. Based on geNorm results at least two genes from among RPS7, RPL30, ACTB or YWHAZ should be chosen for Real Time-PCR result normalization. |
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| Keywords: | Reference gene Endometrium Domestic cat |
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