The effects of EGF and IGF-1 on FSH-mediated in vitro maturation of domestic cat oocytes derived from follicular and luteal stages |
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| Institution: | 1. Department of Obstetrics, Gynaecology and Reproduction, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey;2. Department of Histology and Embriology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey;1. Department of Health, Animal Science and Food Safety, Faculty of Veterinary Medicine, Università degli Studi di Milano, 20133 Milan, Italy;2. Department of Food Science, University of Udine, Udine, Italy;3. Ambulatorio Veterinario Associato Dr. Pellegrini-Rota, Almenno San Bartolomeo, Bergamo, Italy;4. Department of Veterinary Clinical Sciences, University of Teramo, Teramo, Italy;1. Department of Physics, JIS College of Engineering, Block-A, Phase-III, Kalyani, Nadia 741235, India;2. Department of Chemistry, University of Calcutta, 92 A.P.C. Ray Road, Kolkata 700 009, India;1. Robinson Research Institute, and Discipline of Obstetrics and Gynaecology, School of Paediatrics and Reproductive Health, The University of Adelaide, South Australia 5005, Australia;2. Institute of Agriculture, Department of Biological Production, Tokyo University of Agriculture and Technology, Tokyo 183-8509, Japan;3. Follicle Biology Research Group, Vrije Universiteit Brussel, 1090 Brussels, Belgium;4. Mottasis Oy Ltd., Helsinki, Finland;5. School of Women''s and Children''s Health, Discipline of Obstetrics and Gynaecology, University of New South Wales, Barker Street, Randwick, Sydney, New South Wales 2013, Australia;1. Department of Animal Science, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, Turkey;2. Department of Obstetrics and Gynecology, Faculty of Veterinary Medicine, Mehmet Akif Ersoy University, Burdur, Turkey;3. Department of Genetics, Faculty of Veterinary Medicine, Selcuk University, Konya, Turkey;4. Department of Obstetrics and Gynecology, Veterinary Faculty, Near East University, Nicosia; North Cyprus, Mersin, Turkey;5. Department of Biostatistics, Faculty of Medicine, Karabuk University, Karabük, Turkey;6. Centre for Artificial Insemination and Embryo Transfer, University of Veterinary Medicine, Vienna, Austria |
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| Abstract: | The objective of this study was to evaluate the influence of epidermal growth factor (EGF) and insulin like growth factor-I (IGF-1) on the in vitro maturation of cat oocytes recovered from follicular and luteal stage ovaries. Oocytes from follicular (n = 580) and luteal (n = 209) stages were harvested and divided into four groups, which were cultured in FSH-mediated maturation medium supplemented with: (1) EGF alone (25 ng/mL); (2) IGF-1 alone (100 ng/mL); (3) EGF + IGF-1 (25 ng/mL EGF + 100 ng/mL IGF-I); or (4) no growth factor (control). The proportion of follicular stage oocytes reaching the metaphase II stage was significantly higher than that of oocytes obtained at the luteal stage in both control and study groups (p < 0.001). The percentages of oocytes reaching the metaphase II stage during the follicular period were 62.6% in control; 70.9% in EGF; 72.8% in IGF-1, and 78.1% in EGF + IGF-1 groups, whereas the respective values for gametes collected from luteal stage ovaries were 12.5%, 17.5%, 12.5%, and 16.9%. Additionally, the differences between the study and control groups were significant in the case of follicular stage oocytes. Finally, supplementing the maturation medium with EGF and/or IGF-1 significantly enhanced the meiotic maturation of oocytes recovered from follicular stage ovaries. The present study also demonstrated that the combination of EGF and IGF-I provides an additional or synergic effect on meiotic maturation of oocytes recovered from the follicular stage. |
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| Keywords: | EGF IGF-I Cat oocytes |
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