Metabolic flux analysis of recombinant Pichia pastoris growing on different glycerol/methanol mixtures by iterative fitting of NMR-derived 13C-labelling data from proteinogenic amino acids |
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| Affiliation: | 1. Department of Biotechnology, University of Natural Resources and Life Sciences Vienna, Muthgasse 18, Vienna, Austria;2. Bioinformatics Research Center, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea;3. School of Bioengineering, University of Applied Sciences FH Campus Wien, Vienna, Austria;4. Austrian Centre of Industrial Biotechnology, Vienna, Austria;5. Department of Chemistry, University of Natural Resources and Life Sciences, Vienna, Austria;6. Metabolic Engineering National Research Laboratory, Department of Chemical and Biomolecular Engineering (BK21 plus program), BioProcess Engineering Research Center, Center for Systems and Synthetic Biotechnology, KAIST Institute for the BioCentury, Korea Advanced Institute of Science and Technology (KAIST), Daejeon, Republic of Korea;1. Faculty of Applied Biological Sciences, Gifu University, 1-1 Yanagido, Gifu 501-1193, Japan;2. Department of Food Science and Technology, Faculty of Bioindustry, Tokyo University of Agriculture, 196 Yasaka, Abashiri, Hokkaido 099-2493, Japan;1. Faculty of Sciences and Technology, Universidade NOVA de Lisboa, P-2829-516 Caparica, Portugal;2. Instituto de Biologia Experimental e Tecnológica (IBET), Av. da República, EAN, P-2780-157 Oeiras, Portugal;3. Functional Enviromics Technologies S.A., Campus da Caparica, Faculty of Sciences and Technology, Universidade NOVA de Lisboa, P-2829-516 Caparica, Portugal |
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| Abstract: | |
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| Keywords: | α-ketoglutarate Glyceraldehyde-3-phosphate 2-phosphoglycerate 3-phosphoglycerate Glyceraldehyde-3-phosphate peroxisome Dihydroxyacetone Dihydroxyacetone phosphate Eritrose-4-phosphate Ribose-5-phosphate Ribulose-5-phosphate Xylulose-5-phosphate Fructose-6-phosphate Glucose-6-phosphate Sedoheptulose-7-phosphate Fructose-1,6-biphosphate Pyruvate Phosphoenolpyruvate Malate Succinate Citrate ICIT" },{" #name" :" keyword" ," $" :{" id" :" kw0210" }," $$" :[{" #name" :" text" ," _" :" Isocitrate Fumarate Acetyl coenzyme Methanol labelled Methanol non labelled Methanol FullyGly" },{" #name" :" keyword" ," $" :{" id" :" kw0270" }," $$" :[{" #name" :" text" ," _" :" Fully labelled Glycerol CGly" },{" #name" :" keyword" ," $" :{" id" :" kw0280" }," $$" :[{" #name" :" text" ," _" :" non-labelled Glycerol Biomass formation Formaldehyde formate Oxaloacetate Acetalehyde Phosphoribosyl Pirophosphate Methylenetetrahydrofolate Tetrahydrofolate chorismate Inorganic phosphate Inorganic pyrophosphate 1,3-Diphophoglycerate G1P: SUCCoA" },{" #name" :" keyword" ," $" :{" id" :" kw0410" }," $$" :[{" #name" :" text" ," _" :" Succinyl coenzyme A Ketovalerate Dihydrofolate OUR" },{" #name" :" keyword" ," $" :{" id" :" kw0440" }," $$" :[{" #name" :" text" ," _" :" Oxigen Uptake Rate CER" },{" #name" :" keyword" ," $" :{" id" :" kw0450" }," $$" :[{" #name" :" text" ," $$" :[{" #name" :" __text__" ," _" :" CO" },{" #name" :" inf" ," $" :{" loc" :" post" }," _" :" 2" },{" #name" :" __text__" ," _" :" Exchange Rate n.d." },{" #name" :" keyword" ," $" :{" id" :" kw0460" }," $$" :[{" #name" :" text" ," _" :" not determined standard deviation standard error of the mean PPP" },{" #name" :" keyword" ," $" :{" id" :" kw0490" }," $$" :[{" #name" :" text" ," _" :" pentose phosphate pathway BFD" },{" #name" :" keyword" ," $" :{" id" :" kw0500" }," $$" :[{" #name" :" text" ," _" :" biosynthetically directed fractional ROL" },{" #name" :" keyword" ," $" :{" id" :" kw0510" }," $$" :[{" #name" :" text" ," $$" :[{" #name" :" italic" ," _" :" Rhizopus oryzae" },{" #name" :" __text__" ," _" :" lipase NMR" },{" #name" :" keyword" ," $" :{" id" :" kw0520" }," $$" :[{" #name" :" text" ," _" :" Nuclear Magnetic Resonance ETC" },{" #name" :" keyword" ," $" :{" id" :" kw0530" }," $$" :[{" #name" :" text" ," _" :" electron transfer chain |
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