mRNA expression pattern of gonadotropin receptors in bovine follicular cysts |
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| Affiliation: | 1. Laboratorio de Biología Celular y Molecular Aplicada, Facultad de Ciencias Veterinarias, Universidad Nacional del Litoral, Argentina;2. Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Consejo Nacional de Investigaciones Científicas y Tecnológicas, (CONICET), Argentina;1. Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey;2. Department of Pathology, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey;3. Department of Biochemistry, Faculty of Veterinary Medicine, Ankara University, Ankara, Turkey;4. Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, Afyon Kocatepe University, Afyon, Turkey;5. Department of Obstetrics and Gynaecology, Faculty of Veterinary Medicine, İstanbul University, İstanbul, Turkey;6. Veterinary Medicine, Alaca Farm, Bursa, Turkey;1. Faculty of Veterinary Medicine, Department of Obstetrics, Reproduction and Reproductive Disorders, Trakia University, Malka Verea, Student campus, 6000 Stara Zagora, Bulgaria;2. Faculty of Agriculture, Department of Genetics, Breeding and Reproduction, Trakia University, Malka Verea, Student campus, 6000 Stara Zagora, Bulgaria;3. Institute of Biology and Immunology of Reproduction, Department of Immunobiology of Reproduction, Tzarigradsko shose 73, 1113 Sofia, Bulgaria;4. Experimental Station of Agriculture, Bulgaria Square 1, 8300 Sredets, Bulgaria;1. Laboratorio de Biología Celular y Molecular Aplicada, Instituto de Ciencias Veterinarias del Litoral (ICiVet-Litoral), Universidad Nacional del Litoral (UNL)/Consejo Nacional de Investigaciones Científicas y Técnicas (CONICET), Esperanza, Santa Fe, Argentina;2. Facultad de Ciencias Veterinarias del Litoral, Universidad Nacional del Litoral (UNL), Esperanza, Santa Fe, Argentina;3. Centro Universitario Gálvez, Universidad Nacional del Litoral (UNL), Gálvez, Santa Fe, Argentina;1. Artifcial Insemination and Embryo Transfer Dept., Animal Reproduction Research Institute, Agriculture Research Center (ARC), Giza, Egypt;2. Biology of Reproduction Dept., Animal Reproduction Research Institute, Agriculture Research Center (ARC), Giza, Egypt;3. Field Investigation Dept., Animal Reproduction Research Institute, Agriculture Research Center (ARC), Giza, Egypt;1. Laboratorio de Biología Celular y Molecular Aplicada, ICiVet-Litoral (UNL-CONICET), Esperanza, Santa Fe, Argentina;2. Facultad de Ciencias Veterinarias – Universidad Nacional del Litoral, Esperanza, Santa Fe, Argentina;3. Centro Universitario Gálvez (CUG-UNL), Gálvez, Santa Fe, Argentina;1. Department of Animal Science, University of Zanjan, University Blvd, Zanjan45371-38791, Iran;2. Doctor of Veterinary Medicine (DVM), Qazvin Province43867-34166, Iran;3. Zist Dam Group, University Incubator Center, University of Zanjan, University Blvd, Zanjan45371-38791, Iran;4. Department of Reproduction, Obstetrics and Herd Health, Faculty of Veterinary Medicine, Ghent University, Salisburylaan 133, B-9820Merelbeke, Belgium |
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| Abstract: | Follicular growth and steroidogenesis are dependent on gonadotropin binding to their receptors in granulosa and theca cells of ovarian follicles. The aim of the present study was to evaluate the expression patterns of follicle-stimulating hormone receptor (FSHR) and luteinizing hormone receptor (LHCGR) in ovarian follicular structures from cows with cystic ovarian disease (COD) as compared with those of regularly cycling cows. Relative real-time RT-PCR analysis showed that the expression of FSHR mRNA in granulosa cells was highest in small antral follicles, then decreased significantly as follicles increased in size, and was lowest in cysts. FSHR mRNA was not detected in the theca cells of any follicular category, including cysts. LHCGR mRNA expression in granulosa cells was significantly higher in large antral follicles than in cysts, and not detected in granulosa cells of small and medium antral follicles. In theca cells, the expression level of LHCGR mRNA in medium antral follicles was higher than in small and large antral follicles, whereas that in follicular cysts it was similar to those in small and medium antral follicles, but higher than that in large antral follicles. Our findings provide evidence that there is an altered gonadotropin receptor expression in bovine cystic follicles, and suggest that in conditions characterized by altered ovulation, such as COD, changes in the signaling system of gonadotropins may play a fundamental role in their pathogenesis. |
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| Keywords: | Cystic ovary Gonadotropin receptors Cow LH FSH |
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