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Leishmania donovani Infection Enhances Lateral Mobility of Macrophage Membrane Protein Which Is Reversed by Liposomal Cholesterol |
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| Authors: | Moumita Ghosh Koushik Roy Dipanwita Das Mukherjee Gopal Chakrabarti Kingshuk Roy Choudhury Syamal Roy |
| Affiliation: | 1Infectious Diseases and Immunology Division, CSIR-Indian Institute of Chemical Biology, Kolkata, India;2Department of Biotechnology, University of Calcutta, Kolkata, India;3Departmentof Biostatistics and Bioinformatics, Duke University, Durham, North Carolina, United States of America;McGill University, Canada |
| Abstract: | BackgroundThe protozoan parasite Leishmania donovani (LD) reduces cellular cholesterol of the host possibly for its own benefit. Cholesterol is mostly present in the specialized compartment of the plasma membrane. The relation between mobility of membrane proteins and cholesterol depletion from membrane continues to be an important issue. The notion that leishmania infection alters the mobility of membrane proteins stems from our previous study where we showed that the distance between subunits of IFNγ receptor (R1 and R2) on the cell surface of LD infected cell is increased, but is restored to normal by liposomal cholesterol treatment.Conclusions/SignificancesTo our knowledge this is the first direct demonstration that LD parasites during their intracellular life cycle increases lateral mobility of membrane proteins and decreases F-actin level in infected macrophages. Such defects may contribute to ineffective intracellular signaling and other cellular functions. |
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