Membrane glycoproteins of Newcastle disease virus: Nucleotide sequence of the hemagglutinin-neuraminidase cloned gene and structure/function relationship of predicted amino acid sequence |
| |
Authors: | Ana Sagrera César Cobaleda Jose M Gonza´lez De Buitrago Adolfo García-Sastre Enrique Villar |
| |
Institution: | (1) Departamento de Bioquímica y Biología Molecular, Universidad de Salamanca, Plaza Doctores de la Reina s/n, Edificio Departamental, lab 109, E-37007 Salamanca, Spain;(2) Department of Microbiology, Mount Sinai School of Medicine, One Gustave Levy Place, New York, NY 10029-6574, USA |
| |
Abstract: | The nucleotide sequence of the glycoprotein hemagglutinin-neuraminidase (HN) gene of the Newcastle disease virus (NDV) strain Clone-30 has been determined. The open reading frame of the HN gene contains 1731 nucleotides and encodes a protein of 577 amino acids. Three highly conserved patterns among all paramyxovirus HN glycoproteins, and one additional conserved species-specific region are present. The protein contains five potential N-glycosylation sites, all but one located in the C-terminal external domain. The secondary structure prediction shows that the C-terminal external domain is mostly arranged in -sheets, while -helices are predominantly located in the N-terminal domain. The nucleotide sequence data of the HN gene reported in this paper has been deposited in the GenBank database, under accession number AF098289. |
| |
Keywords: | Newcastle disease virus hemagglutinin-neuraminidase glycoprotein viral attachment protein gene sequence structure/function prediction |
本文献已被 SpringerLink 等数据库收录! |
|