Amino acid modifications in canine, equine and porcine pituitary growth hormones, identified by peptide-mass mapping |
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Authors: | Camillo Secchi Anna Berrini Daniela Gaggioli Vitaliano Borromeo |
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Institution: | a Institute of Veterinary Physiology and Biochemistry, University of Milan, Via Celoria 10, 20133 Milan, Italy |
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Abstract: | Modified amino acid residues in porcine, canine and equine growth hormones purified from pituitary glands were characterised by tryptic mapping and high-performance liquid chromatography with on-line coupled electrospray ionisation mass spectrometry (HPLC–ESI-MS) detection. Hormones from all three species showed the same changes. Conversion of Asp128 to iso-Asp128 was a component of native hormones, while deamidation of Asn12 and Asn98 to Asp and iso-Asp, oxidation of Met4, and cyclisation to the pyroglutamyl derivative of Gln139, probably occurred in vitro, during isolation, storage or hydrolysis. Porcine and canine hormones had indistinguishable protein fingerprints, confirming the assumption, based on their cDNA sequences, that their mature primary structures are identical. |
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Keywords: | Amino acids Growth hormones |
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