Improved bioethanol production through simultaneous saccharification and fermentation of lignocellulosic agricultural wastes by <Emphasis Type="Italic">Kluyveromyces marxianus</Emphasis> 6556 |
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| Authors: | Meng Zhang Pratyoosh Shukla Manimaran Ayyachamy Kugen Permaul Suren Singh |
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| Institution: | (1) Department of Biotechnology and Food Technology, Durban University of Technology, P. O. Box 1334, Durban, 4000, South Africa;(2) Department of Biotechnology, Birla Institute of Technology (Deemed University), Mesra, Ranchi, Jharkhand, 835215, India; |
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| Abstract: | The combined effect of simultaneous saccharification and fermentation and separate hydrolysis and fermentation (SHF) for ethanol
production by Kluyveromyces marxianus 6556 was studied using two lignocellulosic feedstocks viz., corncob and soybean cake. The ethanologenic efficiency of K. marxianus 6556 was observed as 28% (theoretical yield) in a fermentation medium containing glucose, but, there was no ethanol production
by cells grown on xylose. A maximum sugar release of 888 mg/g corncob and 552 mg/g soybean cake was achieved through acid
hydrolysis pretreatment. Furthermore, corncob and soybean cake treated with commercial cellulase (100 IU for 48 h) from Trichoderma reesei yielded reducing sugars of 205 and 100 mg/g, respectively. Simultaneous saccharification and fermentation resulted in highest
ethanol production of 5.68 g/l on corncob and 2.14 g/l on soybean cake after 48 h of incubation. On the contrary, the presence
of inhibitors decreased the overall ethanol yield in the hydrolysates obtained through SHF of corncob and soybean cake. |
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