Microbial polyhydroxyalkanote synthesis repression protein PhaR as an affinity tag for recombinant protein purification |
| |
| Authors: | Shuang Zhang Zhi Hui Wang Guo Qiang Chen |
| |
| Affiliation: | (1) Multidisciplinary Research Center, Shantou University, 515063 Shantou, Guangdong, China;(2) Department of Biological Sciences and Biotechnology, School of Life Science, Tsinghua University, 100084 Beijing, China |
| |
| Abstract: | Background PhaR which is a repressor protein for microbial polyhydroxyalkanoates (PHA) biosynthesis, is able to attach to bacterial PHA granules in vivo, was developed as an affinity tag for in vitro protein purification. Fusion of PhaR-tagged self-cleavable Ssp DnaB intein to the N-terminus of a target protein allowed protein purification with a pH and temperature shift. During the process, the target protein was released to the supernatant while PhaR-tagged intein was still immobilized on the PHA nanoparticles which were then separated by centrifugation. |
| |
| Keywords: | |
| 本文献已被 SpringerLink 等数据库收录！ |
|
