Determination of formaldehyde in blood plasma by high-performance liquid chromatography with fluorescence detection |
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| Authors: | Wenhong Luo Hui Li Yuan Zhang Catharina Y W Ang |
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| Institution: | a The Central Laboratory, Shantou University Medical College, Xin Lin Road 22, Shantou City, Guangdong 515031, China;b Department of Health and Human Services, Food and Drug Administration, National Center for Toxicological Research, Division of Chemistry, Jefferson, AR 72079-9502, USA |
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| Abstract: | An HPLC method was developed for the determination of formaldehyde in human blood plasma. The method was based on the determination of the fluorescent product of the chemical reaction between formaldehyde and ampicillin. A 0.2-ml aliquot of blood plasma was reacted directly with ampicillin under acidic and heating conditions. The reaction product was extracted from the matrix with diethyl ether and analyzed by reversed-phase HPLC with fluorescence detection. Recoveries of spiked formaldehyde at the low ppm (μg/ml) level were between 93% and 102% with relative standard deviations less than 8%. The limits of detection and quantitation of formaldehyde in blood plasma samples were 0.46 μg/ml and 0.87 μg/ml, respectively. |
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| Keywords: | Formaldehyde |
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