Histochemical detection of horseradish peroxidase activity in the rat by the vibratome-paraplast method |
| |
| Authors: | R S Liem J T Rokx J D van Willigen |
| |
| Institution: | Orofacial Research Group, School of Dentistry, University of Groningen, The Netherlands. |
| |
| Abstract: | A method is described for the histochemical detection of horseradish peroxidase in Paraplast Plus embedded brain sections. The procedure uses 150-micron-thick Vibratome-cut slices of glutaraldehyde-paraformaldehyde-fixed brain tissue. Tetramethylbenzidine stabilized by diaminobenzidine/cobalt/H2O2 is used as chromogen. The Vibratome-cut slices are dehydrated through a graded series of acetone, cleared in toluol and flat-embedded in Paraplast Plus embedding medium. Serial sections can be cut as thin as 5-7 micron. The method is universal in its application and permits optimal visualization of labeled neurons with great morphological detail at the light-microscopic level. |
| |
| Keywords: | |
|
|
