| C60对体外培养癌细胞的光致作用研究 |
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| 引用本文： | 钱凯先,严庆丰,黄文栋,李文铸.C60对体外培养癌细胞的光致作用研究[J].生物化学与生物物理进展,1997,24(3):237-241. |
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| 作者姓名： | 钱凯先 严庆丰 黄文栋 李文铸 |
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| 作者单位： | 浙江大学生物科学与技术系，杭州 310027;浙江大学生物科学与技术系，杭州 310027;浙江大学生物科学与技术系，杭州 310027;浙江大学物理学系，杭州 310027 |
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| 基金项目： | 浙江省自然科学基金资助项目. |
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| 摘 要： | 研究结果表明用C60-磷脂酰胆碱与体外培养的HeLa细胞融合后，以4 000 lx光强度激发C60(C60浓度20 mg／L)对HeLa细胞具有显著的光致杀伤作用.生化测定证实光激发C60导致膜蛋白巯基含量减少、膜脂过氧化、丙二醛含量增高，SDS-PAGE证明膜蛋白交联，荧光偏振显示膜流动性降低，电镜超微膜结构破坏，经MTT法检测，大部分细胞死亡.C60的强烈光致作用证实了Arbogast等认为光激发C60可产生单线态氧的观态.
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| 关 键 词： | 富勒烯(C60)，癌细胞，自由基，光致作用 |
| 收稿时间： | 1996/5/24 0:00:00 |
| 修稿时间： | 1996/10/27 0:00:00 |
Studies on the Photoaction Effect of C60 on Cancer Cells in vitro and Its Mechanism |
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| QIAN Kaixian,YAN Qingfeng,HUANG Wendong and LI Wenzhu.Studies on the Photoaction Effect of C60 on Cancer Cells in vitro and Its Mechanism[J].Progress In Biochemistry and Biophysics,1997,24(3):237-241. |
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| Authors: | QIAN Kaixian YAN Qingfeng HUANG Wendong and LI Wenzhu |
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| Institution: | Department of Bioscience and Technology, Zhejiang University, Hangzhou 310027, China;Department of Bioscience and Technology, Zhejiang University, Hangzhou 310027, China;Department of Bioscience and Technology, Zhejiang University, Hangzhou 310027, China;Department of Physics, Zhejiang University, Hangzhou 310027, China |
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| Abstract: | After the fusion between the C60-phosphatidylcholine liposome (C60-concentration as 20 mg/L) and HeLa cells and illumination with the Tungsten-Halogen lamp (4 000 lx, 30 min), the most of cells were killed by the evaluation of MTT assay (3-(4,5-dimethythiazol-2-yl)-2,5-diphenyl tetrazolium bromide). Biochemical test showed that photoexcited C60 led to the decrease of the sulfhydryl content of the membrane protein and the increase of the malonidaldehyde (MDA) with the peroxidation of the membrane lipid. SDS-PAGE showed the conjugation of the membrane protein. Fluorescence polarization (FP) indicated that the fluidity of the cell membrane increase. |
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| Keywords: | fullerenes (C60) cancer cell singlet oxygen photodynamics |
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