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好氧反硝化菌的筛选及其脱氮除磷性质的研究
引用本文:于大禹,郭 威,张金榜,张 贺,杨 鹏. 好氧反硝化菌的筛选及其脱氮除磷性质的研究[J]. 微生物学通报, 2009, 36(4): 0598-0603
作者姓名:于大禹  郭 威  张金榜  张 贺  杨 鹏
作者单位:东北电力大学,应用化学与生物工程系,吉林,吉林,132012
基金项目:国家重点基础研究发展计划973计划项目(No. 2007CB206904); 中国电机工程学会电力青年科技创新项目(No. 2008-5); 东北电力大学博士科研启动基金项目(No. BSJXM-200612)
摘    要:利用富集培养基, 从用生活污水驯化后的活性污泥中筛选得到一株具有好氧反硝化兼具除磷功能的细菌。通过形态学及生理生化指标鉴定其为假单胞菌属。利用此好氧反硝化菌处理模拟废水及生活废水, 通过监测总氮、无机磷及CODcr变化确定在C/N摩尔比为3:1、接种量为10%、pH 6.8、30°C条件下处理2 d, 该菌株脱氮、除磷及去除有机物的效果最佳, 活性污泥经此好氧反硝化菌强化后, 对生活废水的处理能力得到明显提升。

关 键 词:好氧反硝化菌   假单胞菌属   脱氮除磷   CODcr   最佳条件

Isolation of an Aerobic Denitrifying Bacterial Strain and Its Characterization in Nitrogen and Phosphorus Removal
YU Da-Yu,GUO Wei,ZHANG Jin-Bang,ZHANG He and YANG Peng. Isolation of an Aerobic Denitrifying Bacterial Strain and Its Characterization in Nitrogen and Phosphorus Removal[J]. Microbiology China, 2009, 36(4): 0598-0603
Authors:YU Da-Yu  GUO Wei  ZHANG Jin-Bang  ZHANG He  YANG Peng
Affiliation:Department of Applied Chemistry and Biological Engineering, Northeast Dianli University, Jilin, Jilin 132012, China;Department of Applied Chemistry and Biological Engineering, Northeast Dianli University, Jilin, Jilin 132012, China;Department of Applied Chemistry and Biological Engineering, Northeast Dianli University, Jilin, Jilin 132012, China;Department of Applied Chemistry and Biological Engineering, Northeast Dianli University, Jilin, Jilin 132012, China;Department of Applied Chemistry and Biological Engineering, Northeast Dianli University, Jilin, Jilin 132012, China
Abstract:Utilizing the enrichment substrate, an aerobic denitrifying bacterial strain with the capability of phosphorus removal was screened from the activated sludge which had been domesticated by actual living sewage. By the morphological observing and identification of the physiological and biochemical indexes, the strain was identified to belong to Pseudomonas. The aerobic denitrifying bacterial strain was applied to deal with the simulated and actual living wastewater. By inspecting the changes of total nitrogen, inorganic phosphorus and CODcr in the wastewater, it was ascertained that the optimal wastewater treatment conditions of the bacterial strain were C/N= 3, inoculated ratio= 10%, pH 6.8, 30°C, treatment time= 2 d. After the activated sludge was strengthened by the aerobic denitrifying bacterial strain, its treatment capability for actual living sewage was improved, evidently.
Keywords:Aerobic denitrifying bacteria   Pseudomonas   Nitrogen and phosphorus removal   CODcr   Optimal conditions
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