Human microvessel endothelial cells: Isolation,culture and characterization |
| |
Authors: | P W Hewett J C Murray |
| |
Institution: | (1) Endothelial Biology Group, CRC Gray Laboratory, Mount Vernon Hospital, P.O. Box 100, HA6 2JR Northwood, Middlesex, United Kingdom |
| |
Abstract: | Summary Over recent years, interest in endothelial cell biology has increased dramatically with our ability to grow and study endothelial
cellsin vitro. While large veins and arteries remain a quick and convenient source of endothelial cells, the great morphological, biochemical
and functional heterogeneity that endothelial cells express has necessitated the development of techniques to isolate microvessel
endothelial cells from different tissues to create more realisticin vitro models. The majority of isolation procedures employ selective methods to enrich microvessel endothelial cells from tissue
homogenates directly, or after a period in culture. These include sieving/filtration, manual weeding, isopycnic centrifugation,
selective growth media, and the use of flow cytometry or magnetic beads coupled with specific endothelial cell markers. The
establishment of pure endothelial cell populations is important for studying their biochemistry and physiology and there are
many morphological, immunological and biochemical criteria which can be used to characterize human endothelial cells. These
range from classical markers such as von Willebrand Factor and angiotensin-converting enzyme to novel markers like platelet
endothelial cell adhesion molecule-1 (CD31) and the expression of E-selectin on cytokine-activated endothelial cells. |
| |
Keywords: | Microvessel endothelium isolation techniques characterization cell markers antibodies |
本文献已被 SpringerLink 等数据库收录! |
|