Cystic fibrosis as a bowel cancer syndrome and the potential role of CK2 |
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Authors: | Anil Mehta |
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Institution: | (1) Department of Endocrinology, Xin Hua Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200092, P.R. China;(2) Shanghai Institute of Endocrinology and Metabolism, Rui Jin Hospital, Shanghai Jiaotong University School of Medicine, Shanghai, 200025, P.R. China |
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Abstract: | Thyroid hormones are major regulators of postnatal brain development. Thyroid hormones act through nuclear receptors to modulate
the expression of specific genes in the brain. We have used microarray analysis to identify novel responsive genes in 14-day-old
hypothyroid rat brains, and discovered that synaptosomal-associated protein of 25 kDa (SNAP-25) was one of the thyroid hormone-responsive
genes. SNAP-25 is a presynaptic plasma membrane protein and an integral component of the vesicle docking and fusion machinery
mediating secretion of neurotransmitters and is required for neuritic outgrowth and synaptogenesis. Using microarray analysis
we have shown that SNAP-25 was down-regulated in the hypothyroid rat brain compared with the age-matched controls. Real-time
RT-PCR and western blotting analysis confirmed that SNAP-25 mRNA and protein levels decreased significantly in the developing
hypothyroid rat brain. Our data suggest that in the developing rat brain, SNAP-25 expression is regulated by thyroid hormone,
and thyroid hormone deficiency can cause decreased expression of SNAP-25 and this may on some level account for the impaired
brain development seen in hypothyroidism. |
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Keywords: | Thyroid hormone Thyroid hormone-responsive gene Hypothyroidism Microarray Brain Development SNAP-25 Gene expression |
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