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| 48株临床分离假丝酵母菌DNA异质性分析 | |
| 引用本文： | 李菁,张文莉,郭薇媛,张和光,马佳毓. 48株临床分离假丝酵母菌DNA异质性分析[J]. 微生物学杂志, 2006, 26(4): 91-94 |
| 作者姓名： | 李菁 张文莉 郭薇媛 张和光 马佳毓 |
| 作者单位： | 1. 齐齐哈尔卫生学校,微生物教研室,黑龙江,齐齐哈尔,161006 2. 哈尔滨医科大学,黑龙江,哈尔滨,150086 3. 哈尔滨医科大学,附属二院,黑龙江,哈尔滨,150086 |
| 摘 要： | 应用随机扩增多态性DNA技术(RAPD)对48株假丝酵母菌临床菌株进行PCR扩增,并对扩增产物的指纹图谱进行分析,结果显示:48株假丝酵母菌中白假丝酵母菌35株,非白假丝酵母菌13株,引物1和引物2将来源不同的35株白假丝酵母菌分成4型和6型,且RAPD技术可将白假丝酵母菌鉴定至种,并可分辩同种菌的不同型别,是假丝酵母菌致感染、追踪病原的分子流行病学研究有效方法。 |
| 关 键 词： | 假丝酵母菌 随机扩增DNA多态性 |
| 文章编号： | 1005-7021(2006)04-0091-04 |
| 修稿时间： | 2006-04-03 |
DNA Heterogeneity Analysis of 48 Clinic-Isolated Candida Strains |
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| LI Jing,ZHANG Wen-li,GUO Wei-yuan,ZHANG He-guang,MA Jia-yu. DNA Heterogeneity Analysis of 48 Clinic-Isolated Candida Strains[J]. Journal of Microbiology, 2006, 26(4): 91-94 | |
| Authors: | LI Jing ZHANG Wen-li GUO Wei-yuan ZHANG He-guang MA Jia-yu |
| Affiliation: | 1.Teach.& Res.Div.of Microbiol.Qiqihar Health Sch.Qiqihar 161006;2.Harbin Med.Univ.Harbin 150085;3.Second Affiliated Hosp.Harbin Med.Univ. Harbin 150086 |
| Abstract: | Genotypic DNA of 48 clinic-isolated Candida strains were amplified with RAPD(Random primer of amplified polymorphism DNA),and analyzed their fingerprints.The results revealed that they consisted of 35 Candida albicans strains and 13 non-C.albicans strains.The 35 C.albicans isolated could be classified into 4 and 6 genotype by the primer 1 and primer 2.RAPD method not only identifies Candida species,but also distinguishes further different type in the same species,and is a fine molecular epidemiological method to investigate nosocomial infection. |
| Keywords: | Candida RAPD |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |