16SrRNA sequencing of Dye decolorizing bacteria isolated from Soil

Dye׳s residues in textile effluents are hazardous for humans and animals health. Such pollutants can be degraded intonon-harmful molecules using biological approaches that are considered cheaper and ecologically safer. Isolated 15 bacterialcultures from soil that could be used in biological system were showed decolorization capacity for Acid Green dye (33.9% to94.0%) using thin layer chromatography and broth culture method. The most promising cultures (AMC3) to decolorizeAcid green Dye (94.6%) was re-coded as NSDSUAM for submitting at IMTECH, Chandigarh for sequencing. The 16SrRNAsequencing suggested that it can be a variant of Pseudomonas geniculata (99.85% identical similarity) with difference of 2 base pairsto reference strain Pseudomonas geniculata ATCC 19374(T). Thus present study proposed dye decolorizing efficiency of theisolated strain of Pseudomonas geniculata that was previously unnoticed. The sequence is deposited in NCBI GenBank with theaccession number {"type":"entrez-nucleotide","attrs":{"text":"KP238100","term_id":"745856746","term_text":"KP238100"}}KP238100.