| Abstract: | An automatable column switching technique for extraction of cyclooxygenase and lipoxygenase products has been developed. The extraction column is a 0.4 cm X 3 cm stainless steel column slurry packed with Polygosil (C18, 25-40 mu particle diameter). Supernatants of incubations terminated by the addition of 1 volume MeOH, bath sonicated and centrifuged were pumped onto the extraction column. The non-adherent polar material was eluted to waste with up to five times the sample volume with a high polarity mobile phase. Following this, the extraction column was then eluted to an analytical column with a mobile phase of decreased polarity. |
