| 旋毛虫Ts87抗原的免疫学特性及保护性的初步研究 |
| |
| 引用本文： | 杨静,诸欣平,杨雅平,雷丽萍.旋毛虫Ts87抗原的免疫学特性及保护性的初步研究[J].动物学杂志,2003,38(3):52-55. |
| |
| 作者姓名： | 杨静 诸欣平 杨雅平 雷丽萍 |
| |
| 作者单位： | 首都医科大学基础医学院寄生虫学教研室,北京,100054 |
| |
| 基金项目： | 美国中华医学会资助项目，北京市教委科技发展计划项目，北京市跨世纪优秀人才工程项目，Parasitology 98-674，2001-2004，01KJ-091，，， |
| |
| 摘 要： | 应用Western btot和ELISA方法对纯化的重组蛋白PET—28a( )／Ts87进行免疫学特性鉴定及保护性研究。Western blot和ELISA结果显示，Ts87抗原可被人工感染旋毛虫的兔血清、病猪血清、病人血清及抗Ts87的兔血清所识别。Ts87抗原免疫BABL/c小鼠，较对照组减虫率为29％，说明Ts87抗原可作为旋毛虫免疫诊断和疫苗的候选抗原。
|
| 关 键 词： | 旋毛形线虫 Ts87抗原 诊断 保护性免疫 ELISA |
| 文章编号： | 0250-3263(2003)03-52-04 |
Immunological Characteristics and Protective Immunity of the Trichinella spiralis Ts87 Antigen |
| |
| YANG Jing,ZHU Xin-Ping,YANG Ya-Ping,LEI Li-Ping.Immunological Characteristics and Protective Immunity of the Trichinella spiralis Ts87 Antigen[J].Chinese Journal of Zoology,2003,38(3):52-55. |
| |
| Authors: | YANG Jing ZHU Xin-Ping YANG Ya-Ping LEI Li-Ping |
| |
| Abstract: | The immunological characteristics and protective immunity of the purified recombinant protein Ts87 were analyzed by Western blot and ELISA. The Ts87 antigen could be recognized by infected rabbit sera, infected swine sera, infected human sera and antisera against Ts87 by Western blot and ELISA. The immunoprotective effect of the Ts87 antigen of Trichinella spiralis was studied by a challenge experiment. Compared with a control group, numbers of larvae in the muscle of immunized mice decreased by 29%. The results showed that the Ts87 antigen might be both a promising immunodiagnosis antigen and a vaccine candidate for Trichinella spiralis. |
| |
| Keywords: | Trichinella spiralis Ts87 antigen Diagnosis Protective immunity ELISA |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《动物学杂志》浏览原始摘要信息 |
| 点击此处可从《动物学杂志》下载免费的PDF全文 |
