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Chromosome numbers, karyotypes and nuclear DNA contents from perennial polyploid groups ofCerastium (Caryophyllaceae)
Authors:Monica Boşcaiu  Oscar Vicente  Friedrich Ehrendorfer
Institution:(1) Institut für Botanik, Universität Wien, Rennweg 14, A-1030 Wien, Austria;(2) Present address: Jardí Botànic de València, Beat Gaspar de Bono s/n, E-46008, Valencia, Spain;(3) Institut für Mikrobiologie und Genetik, Wiener Biozentrum, Dr. Bohr-Gasse 9, A-1030 Wien, Austria;(4) Present address: Instituto de Biología Molecular y Celular de Plantas, Universidad Politècnica de Valencia, Camino de Vera s/n, E-46022 Valencia, Spain;(5) Institut für Botanik, Universität Wien, Rennweg 14, A-1030 Wien, Austria;(6) Kommission für Interdisziplinäre Ökologische Studien, Österreichischen Akademie der Wissenschaften, Austria
Abstract:Somatic chromosome numbers have been determined for the followingCerastium taxa:C. eriophorum (2n = 36),C. alpinum (2n = 72),C. transsylvanicum (2n = 108),C. arcticum (2n = 108),C. latifolium (2n = 36),C. carinthiacum (2n = 36),C. banaticum (2n = 36),C. arvense subsp.glandulosum (2n = 36),C. arvense subsp.arvense (2n = 72) andC. fontanum (2n = 144). Karyotypes of three diploid species (C. eriophorum, C. banaticum andC. latifolium), belonging to three different taxonomic groups, were analysed and found to be similar. The relative nuclear DNA contents of all taxa were determined by flow cytometry and, for five species, also by Feulgen cytophotometry. The values obtained by the two methods are similar. A comparison of nuclear DNA contents among diploids shows that values differ significantly between different taxonomic groups, and are correlated with average chromosome size. Within closely related polyploid groups nuclear DNA amounts increase from 2x- to 4x- and 6x taxa as 1 : 1.4 : 2.4 in theC. alpinum complex, whereas DNA amounts are doubled comparing 2x- and 4x-subspecies in theC. arvense complex.
Keywords:Caryophyllaceae  Cerastium  Chromosome numbers  nuclear DNA content  flow cytometry  Feulgen cytophotometry
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