Influence of a Mechanical Tension on the B-C and B-C Conformational Transitions in DNA Fibres |
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Authors: | G Albiser M Harmouchi S Premilat |
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Institution: | Laboratoire de Biophysique Moléculaire, U.A. C.N.R.S. N° 494 Faculté des Sciences , Université de Nancy , I., B.P. N° 239, 54506 , Vandoeuvre les Nancy , France |
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Abstract: | Abstract In the present fibre X-ray study we attempt to quantify the effect of a mechanical tension on the conformations, and transitions between the structural forms of DNA A simple experimental device has been realized in order to apply precise mechanical forces on DNA fibres during X-ray exposure. It is shown that, as the applied tension is increased, the B→A transition can be prevented as well as with a decrease of the sodium salt content A kind of distorted B form is then observed the helical parameters of which change with the relative humidity. On the contrary, the mechanical tension does not prevent the B→C transition; it only slows down the form change and improves the X-ray patterns up to a relative humidity of 0%. |
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