A Study of the B-A Transition in DNA by Gel Electrophoresis |
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| Authors: | L. S. Shlyakhtenko |
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| Affiliation: | Institute of Molecular Genetics, Academy of Sciences of the USSR , Moscow , 123182 , USSR |
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| Abstract: | Abstract A procedure is developed for studying the B-A transition in DNA using gel electrophoresis. The starting point has been the idea that the junction between the A and B sections, which appear within the transition interval would increase the mobility of the DNA molecules. Indeed, the mobility of DNA in a gel is shown to increase in the middle of the B-A transition due to the formation of the largest possible number of boundaries between the B and A forms. The middle of the B-A transition in supercoiled DNA appears to be shifted against the middle of the transition in open circular (as well as linear) DNA by about 1.3% towards lower ethanol concentrations under the influence of the superhelical stress. |
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