48 NMR study of the interaction between G-quadruplexes and small molecules |
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Authors: | Wan Jun Chung |
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Institution: | School of Physical and Mathematical Sciences, Nanyang Technological University , Singapore , 637371 , Singapore Phone: Phone: (+65) 6514 1915 Fax: Phone: (+65) 6514 1915 |
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Abstract: | Guanine-rich oligonucleotides are able to adopt secondary DNA structures, known as G-quadruplexes. Such G-rich sequences are found in human telomeres, promoter regions of oncogenes, 5′ untranslated regions (UTRs) of mRNAs and human intronic sequences. Studies have shown that small molecules can induce anti-cancer effect through stabilizing or promoting G-quadruplex formation. In order to design and develop a potent drug, structural details on the interaction between small molecules and G-quadruplexes are invaluable. In this study, we seek to understand the structural determinants involved in the interaction between G-quadruplexes and small molecules. NMR spectroscopy is employed to resolve the structures of two intramolecular G-quadruplexes bound to small molecules. These resolved complexes allow us to structurally design new potent drugs for their application in anti-cancer therapy. |
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