The Formation Of Parallel RNA-RNA Duplexes In Vitro |
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| Authors: | Nickolai A. Tchurikov Natalia A. Ponomarenko Yulia B. Golova Boris K. Chernov |
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| Affiliation: | 1. Department of Genome Organization;2. University of Oslo, Center for Medical Studies , Moscow , Vavilov str.32, Russia;3. Group of Genes Chemical Synthesis , Engelhardt Institute of Molecular Biology, Russian Academy of Sciences , Vavilov str. 32, Moscow , B334, l17984 , Russia |
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| Abstract: | Abstract Over the years the structural properties of nucleic acids have been of interest, providing data that may be of importance for DNA and RNA organization and function in the cell. We have attempted to look for the formation of parallel RNA-RNA duplexes in vitro. RNA molecules comprising complementary in the same polarity alternating stretches of A and U of increasing length were enzymatically synthesized and annealed in physiological conditions. The fractionation in the denaturing Polyacrylamide gels revealed the formation of two types of full-length parallel RNase A - stable duplexes established either by A-U or by A-A and U-U self pairs. These results suggest novel structural properties of versatile RNA molecules that potentially may be realized in vivo. |
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