Revealing of the Spin-Spin Interaction Due to Incorporating a Spin Label in the Carbohydrate Parts of Immuniglobulins M and G |
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Authors: | V P Timofeev D O Nikolsky V A Lapuk V A Alyoshkin |
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Institution: | 1. V. A. Engelhardt Institute of Molecular Biology Russian Academy of Sciences , Moscow , 117984 , Russian Federation;2. N. D. Zelinsky Institute of Organic Chemistry Russian Academy of Sciences , Moscow , 119991 , Russian Federation;3. State Institution “Moscow G. N. Gabrichevsky Scientific- Research Institute of Epidemiology and Microbiology, Public Health Ministry of Russia” , Moscow , 125212 , Russian Federation |
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Abstract: | Abstract The EPR spectra of the preparations produced by spin labeling of the carbohydrate parts in monoclonal IgM and normal IgG with 2,2,6,6-tetramethyl-4-aminopiperidine-1-oxyl as the spin label indicate the existence of a rapid spin-spin exchange interaction between two spin labels. In the case of spin-labeled IgM, the carrier of such a spectrum is shown to be a glycopeptide noncovalently bound to IgM; it includes two spin labels and may be detached from the macromolecule by a combination of dialysis and gel filtration. |
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