Control Region of SV40 Minichromosomes is Preferentially Cleaved by Single-Strand Specific SI Nuclease |
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| Authors: | S A Nedospasov A N Shakhov B Hirt |
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| Institution: | 1. Swiss Institute for Experimental Cancer Research , CH-1066 , Epalinges , Switzerland;2. Institute of Molecular Biology USSR Academy of Sciences , B-334 , Moscow , USSR;3. Institute of Molecular Biology USSR Academy of Sciences , B-334 , Moscow , USSR;4. Swiss Institute for Experimental Cancer Research , CH-1066 , Epalinges , Switzerland |
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| Abstract: | Abstract We have analysed SI sensitivity of SV40 minichromosomes isolated from the nuclei of infected cells at the late stage of infection. We show that a fraction of purified minichromosomes is sensitive towards double-strand cleavage by SI nuclease. The pattern of specific cleavage reminiscent ofthat found for subcloned fragment under supercoiling is superimposed upon apparently random double-strand cuts along the entire regulatory region. Therefore, the cleavage sites are not exclusively confined to the regions with the reported alternate DNA conformation. |
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