Yeast tRNA Asp-Aspartyl-tRNA Synthetase: The Crystalline Complex

Abstract

Aspartyl-tRNA synthetase from yeast, a dimer of molecular weight 125,000 and its cognate tRNA (Mr=24,160) were co-crystallized using ammonium sulfate as precipitant agent. The presence in the crystals of both components in the two-to-one stoichiometric ratio was demonstrated by electrophoresis, biological activity assays and crystallographic data. Crystals belong to the cubic space group 1432 with cell parameter of 354 Å and one complex particle per asymmetric unit.

The solvent content of about 78% is favorable for a low resolution structural investigation. By exchanging H₂O for D₂O in mother liquors, advantage can be taken from contrast variation techniques with neutron radiations. Diffraction data to 20 Å resolution were measured at five different contrasts, two of them being close to the theoretical matching point of RNA and protein in the presence of ammonium sulfate. The experimental extinction of the diffracted signal was observed to be close to 36% D₂O, significantly different from the predicted value of 41%. The phenomenon can be explained by the existence of a large interface region between the two tRNAs and the enzyme. These parts of the molecules are hidden from the solvent and their protons are less easily exchangeable. Accessibility studies toward chemicals of tRNA^Asp in solution and in the presence of synthetase are in agreement with such a model.