Evolutionary perspective on the origin of Haitian cholera outbreak strain |
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Authors: | Anirban Dasgupta Rachana Banerjee Santasabuj Das |
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Affiliation: | 1. Department of Bioinformatics , West Bengal University of Technology , BF-142, Sector – I, Salt Lake, Kolkata , 700064 , India;2. Department of Bio-Physics, Molecular Biology and Bioinformatics , University of Calcutta , 92, Acharya Prafulla Chandra Road, Kolkata , 700009 , India;3. Biomedical Informatics Center, National Institute of Cholera and Enteric Diseases , P-33, C.I.T Road, Scheme-XM, Beliaghata, Kolkata , 700010 , India |
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Abstract: | Cholera epidemic has not been reported in Haiti for at least 100?years, although cholera has been present in Latin America since 1991. Surprisingly, the recent cholera epidemic in Haiti (October 2010) recorded more than 250,000 cases and 4000 deaths in the first 6?months and became one of the most explosive and deadly cholera outbreak in recent history. In the present study, we conducted genomic analyses of pathogenicity islands of three Haitian Vibrio cholerae strains and compared them with nine different V. cholerae O1 El Tor genomes. Although CIRS101 is evolutionarily most similar to the Haitian strains, our study also provides some important differences in the genetic organization of pathogenicity islands of Haitian strains with CIRS101. Evolutionary analysis suggests that unusual functional constraints have been imposed on the Haitian strains and we hypothesize that amino acid substitution is more deleterious in Haitian strains than in nonHaitian strains. |
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Keywords: | Cholera Haitian outbreak pathogenicity islands comparative genomics functional constraints |
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