Natural Occurrence of Left-Handed (Z) Regions in PM2 DNA |
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| Authors: | F D Miller K F Jorgenson R J Winkfein J H van de Sande D A Zarling J Stockton |
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| Institution: | 1. Department of Medical Biochemistry , The University of Calgary , Calgary , Alberta , Canada , T2N 4N1;2. Max Planck Institute fur Biophysikalische Chemie , Postfach 958, D-3400 , Goettingen , FRG;3. Department of Anatomy , The University of Calgary , Calgary , Alberta , Canada , T2N 4N1 |
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| Abstract: | Abstract Bacteriophage PM2 DNA, a ccc genome of high apparent superhelical density, contains left-handed (Z) regions as detected by competitive radioimmunoassay, agarose gel electrophoresis of DNA: antibody complexes and immunoelectron microscopy. The latter technique, in conjunction with partial blockage of restriction endonuclease sites by bound antibody, was used to map the left-handed regions along the DNA molecule. A cluster of four to five antibody molecules (approximately 25% of bound antibody) was located within map units 0.05–0.18 of the single Hpa II restriction site. Sequence analysis of part of this region showed the presence of several areas of high alternating purine-pyrimidine content. A strong correlation is observed between alternating pyrimidine-purine tracts of significant length and antibody binding sites. |
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